Viral Capsid D•Dr. Bob Wilson

Glow Discharge

Room Temp
Make grid hydrophilic
40 seccompleted

Sample Application

4°C / 100% Humidity
Apply 3µl of protein
Instantcompleted

Blotting

4°C
Remove excess liquid
3-6 secfailed

Plunge Freeze

-180°C
Rapid cooling in Ethane
Microsecondspending

Grid Storage

-196°C
Transfer to LN2
Indefinitepending

Vitrification (Plunge Freezing)

Viral Capsid D

Cryo-EM
10 mins (aborted)

Protocol Details

  • Completed successfully.
TempRoom Temp
Duration40 sec
Execution Times
Started: 12/19/2024, 11:00:00 AM
Finished: 12/19/2024, 11:00:40 AM