Membrane Protein C•Dr. Alice Johnson

Glow Discharge

Room Temp
Make grid hydrophilic
45 seccompleted

Sample Application

4°C / 100% Humidity
Apply 3µl of protein
Instantcompleted

Blotting

4°C
Remove excess liquid
4 seccompleted

Plunge Freeze

-180°C
Rapid cooling in Ethane
Microsecondscompleted

Grid Storage

-196°C
Transfer to LN2
Indefinitecompleted

Vitrification (Plunge Freezing)

Membrane Protein C

Cryo-EM
55 mins

Protocol Details

  • Plasma treatment completed successfully.
TempRoom Temp
Duration45 sec
Execution Times
Started: 12/18/2024, 2:00:00 PM
Finished: 12/18/2024, 2:00:45 PM