Antibody Complex A•Dr. Jane Smith

Glow Discharge

Room Temp
Make grid hydrophilic
30-60 seccompleted

Sample Application

4°C / 100% Humidity
Apply 3µl of protein
Instantcompleted

Blotting

4°C
Remove excess liquid
3-6 seccompleted

Plunge Freeze

-180°C
Rapid cooling in Ethane
Microsecondscompleted

Grid Storage

-196°C
Transfer to LN2
Indefinitecompleted

Vitrification (Plunge Freezing)

Antibody Complex A

Cryo-EM
45 mins

Protocol Details

  • Uses plasma to clean carbon grids.
  • Makes the surface negatively charged and hydrophilic.
  • Ensures even spreading of the sample solution.
TempRoom Temp
Duration30-60 sec
Execution Times
Started: 12/15/2024, 9:00:00 AM
Finished: 12/15/2024, 9:01:00 AM